Drosophila_11D

Sample information

Picture
Location
Collection date 04/14/2022
Captive / Cultivated? Captive / Cultivated
Group The Summit Country Day School
Observations

From Carolina Biological, Stock 3/23

-Drosophila were Miniature

Putative identification Arthropoda Hexapoda Insecta Diptera Drosophilidae Drosophila Drosophila melanogaster

Methods

Extraction kit Thermoscientific GeneJet Genomic Purification
DNA extraction location Whole arthropod
Single or Duplex PCR Duplex Reaction
Gel electrophoresis system MiniPCR
Buffer TBE
DNA stain GelGreen
Gel images
Protocol notes

Results

Wolbachia presence No
Confidence level High
Explanation of confidence level

Our positive, negative, and water controls all worked and yielded the expected results.

Wolbachia 16S sequence
Arthropod COI sequence
Summary The Drosophila melanogaster was found to be negative for Wolbachia.
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