Sample information |
|
| Picture |
|
|---|---|
| Location | |
| Collection date | 04/11/2022 |
| Captive / Cultivated? | Captive / Cultivated |
| Group | The Summit Country Day School |
| Observations | These are cultured fruit flies purchased by Carolina Biological and arrived on March 23, 2022. The strain was miniature apterous. |
| Putative identification | Arthropoda Hexapoda Insecta Diptera Drosophilidae Drosophila Drosophila melanogaster |
Methods |
|
| Extraction kit | Thermoscientific GeneJet Genomic Purification |
| DNA extraction location | Whole arthropod |
| Single or Duplex PCR | Duplex Reaction |
| Gel electrophoresis system | MiniPCR |
| Buffer | TBE |
| DNA stain | GelGreen |
| Gel images |
|
| Protocol notes | |
Results |
|
| Wolbachia presence | No |
| Confidence level | High |
| Explanation of confidence level | We are confident in our controls. For our positive control showed the two genes being expressed while the negative control only had one one gene, the COI. The water control also proved further our procedure was thorough for it did not have any genes. |
| Wolbachia 16S sequence | |
| Arthropod COI sequence |
|
| Summary | The Drosophila melanogaster was found to be negative for Wolbachia. |
Water Boatman