Pavement ant

Sample information

Photos by: James H.
Collection date 04/13/2022
Captive / Cultivated? Wild-caught
Group Hampton Roads Academy

On pavement, small black ant

Putative identification Arthropoda Hexapoda Insecta Hymenoptera Formicidae Tetramorium Tetramorium immigrans


Extraction kit Edwards Buffer
DNA extraction location Whole arthropod
Single or Duplex PCR Single Reaction
Gel electrophoresis system MiniOne
Buffer TBE
DNA stain GelGreen
Gel images
Protocol notes

DNA pellet was a light purple color, no difficulties in the lab.


Wolbachia presence No
Confidence level Medium
Explanation of confidence level

The test was clearly negative. Positive control didn’t show an arthropod band.

Wolbachia 16S sequence
Arthropod COI sequence
Summary The Tetramorium immigrans was found to be negative for Wolbachia.
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