Sample information |
|
| Picture |
|
|---|---|
| Location | |
| Collection date | 04/13/2022 |
| Captive / Cultivated? | Wild-caught |
| Group | Hampton Roads Academy |
| Observations | On pavement, small black ant |
| Putative identification | Arthropoda Hexapoda Insecta Hymenoptera Formicidae Tetramorium Tetramorium immigrans |
Methods |
|
| Extraction kit | Edwards Buffer |
| DNA extraction location | Whole arthropod |
| Single or Duplex PCR | Single Reaction |
| Gel electrophoresis system | MiniOne |
| Buffer | TBE |
| DNA stain | GelGreen |
| Gel images |
|
| Protocol notes | DNA pellet was a light purple color, no difficulties in the lab. |
Results |
|
| Wolbachia presence | No |
| Confidence level | Medium |
| Explanation of confidence level | The test was clearly negative. Positive control didn’t show an arthropod band. |
| Wolbachia 16S sequence | |
| Arthropod COI sequence |
|
| Summary | The Tetramorium immigrans was found to be negative for Wolbachia. |
Water Boatman