R.Stanley’s Bug

April 27, 2022

Sample information
Picture
Photos by: Rebekah S
Location
Collection date 03/30/2022
Captive / Cultivated? Wild-caught
Group Richmond High School
Observations

Was curled up when caught
Putative identification Arthropoda Myriapoda Diplopoda

Methods
Extraction kit Thermoscientific GeneJet Genomic Purification
DNA extraction location Abdomen
Single or Duplex PCR Duplex Reaction
Gel electrophoresis system Standard electrophoresis system
Buffer TAE
DNA stain SYBR Safe
Gel images
Protocol notes

We did everything correctly

Results
Wolbachia presence Unknown
Confidence level High
Explanation of confidence level

Nothing showed up in my lane/
Wolbachia 16S sequence
Arthropod COI sequence
Summary
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