Scarab Beetle

Sample information

Collection date 04/14/2022
Captive / Cultivated? Wild-caught
Group Hampton Roads Academy


-visually matches a beetle

-hard exoskeleton


Putative identification Arthropoda Hexapoda Insecta Coleoptera


Extraction kit Edwards Buffer
DNA extraction location Abdomen
Single or Duplex PCR Single Reaction
Gel electrophoresis system MiniOne
Buffer TBE
DNA stain GelGreen
Gel images
Protocol notes

-Dissecting abdomen was a bit confusing

-Injecting dna into gel was a little difficult

-Dna was brown and stuck to the bottom of tlhe vial


Wolbachia presence No
Confidence level Medium
Explanation of confidence level

This was my first time doing this lab, and I don’t understand the results very much, so I’m not sure, if I did it properly.

Negative control showed Wolbachia

Wolbachia 16S sequence
Arthropod COI sequence
Summary The Coleoptera was found to be negative for Wolbachia.
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