Sample information |
|
| Picture |
|
|---|---|
| Location | |
| Collection date | 04/14/2022 |
| Captive / Cultivated? | Wild-caught |
| Group | Hampton Roads Academy |
| Observations | -black -visually matches a beetle -hard exoskeleton -wings |
| Putative identification | Arthropoda Hexapoda Insecta Coleoptera |
Methods |
|
| Extraction kit | Edwards Buffer |
| DNA extraction location | Abdomen |
| Single or Duplex PCR | Single Reaction |
| Gel electrophoresis system | MiniOne |
| Buffer | TBE |
| DNA stain | GelGreen |
| Gel images |
|
| Protocol notes | -Dissecting abdomen was a bit confusing -Injecting dna into gel was a little difficult -Dna was brown and stuck to the bottom of tlhe vial |
Results |
|
| Wolbachia presence | No |
| Confidence level | Medium |
| Explanation of confidence level | This was my first time doing this lab, and I don’t understand the results very much, so I’m not sure, if I did it properly. Negative control showed Wolbachia |
| Wolbachia 16S sequence | |
| Arthropod COI sequence |
|
| Summary | The Coleoptera was found to be negative for Wolbachia. |
Water Boatman