Beetle – ABH1

September 7, 2023

Sample information
Picture
Location
Collection date 05/02/2022
Captive / Cultivated? Captive / Cultivated
Group Woodford County High School
Observations

Black beetle shell with a dark black body. The beetle has very skinny legs with a yellow circle hanging off its body.
Putative identification Arthropoda Hexapoda Insecta Coleoptera

Methods
Extraction kit Instagene Matrix
DNA extraction location Whole arthropod
Single or Duplex PCR Single Reaction
Gel electrophoresis system Standard electrophoresis system
Buffer TAE
DNA stain Fast Blast
Gel images
Protocol notes

Results
Wolbachia presence No
Confidence level Medium
Explanation of confidence level
Wolbachia 16S sequence
Arthropod COI sequence
Summary The Coleoptera was found to be negative for Wolbachia.
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