Sample information
Picture	Photos by: MNT
Location
Collection date	05/02/2022
Captive / Cultivated?	Captive / Cultivated
Group	Woodford County High School
Observations	smll, long legs, dark, small body.
Putative identification	Arthropoda Hexapoda Insecta Diptera
Methods
Extraction kit	Instagene Matrix
DNA extraction location	Whole arthropod
Single or Duplex PCR	Single Reaction
Gel electrophoresis system	Standard electrophoresis system
Buffer	TAE
DNA stain	Fast Blast
Gel images
Protocol notes
Results
Wolbachia presence	No
Confidence level	Medium
Explanation of confidence level
Wolbachia 16S sequence
Arthropod COI sequence
Summary	The Diptera was found to be negative for Wolbachia.