Sample information
Picture
Location
Collection date	05/06/2022
Captive / Cultivated?	Wild-caught
Group	Woodford County High School
Observations
Putative identification	Arthropoda Hexapoda Insecta Diptera Ceratopogonidae
Methods
Extraction kit	Instagene Matrix
DNA extraction location	Whole arthropod
Single or Duplex PCR	Single Reaction
Gel electrophoresis system	Standard electrophoresis system
Buffer	PBS
DNA stain	Fast Blast
Gel images
Protocol notes
Results
Wolbachia presence	No
Confidence level	High
Explanation of confidence level	There was no sign of being positive for wolbachia
Wolbachia 16S sequence
Arthropod COI sequence
Summary	The Ceratopogonidae was found to be negative for Wolbachia.