Sample information |
|
| Picture |
|
|---|---|
| Location | |
| Collection date | 05/16/2022 |
| Captive / Cultivated? | Wild-caught |
| Group | Woodford County High School |
| Observations | blue-green beetle, mandibles, six white spots, |
| Putative identification | Arthropoda Hexapoda Insecta Coleoptera |
Methods |
|
| Extraction kit | Instagene Matrix |
| DNA extraction location | Whole arthropod |
| Single or Duplex PCR | Single Reaction |
| Gel electrophoresis system | Standard electrophoresis system |
| Buffer | TAE |
| DNA stain | Fast Blast |
| Gel images |
|
| Protocol notes | |
Results |
|
| Wolbachia presence | Yes |
| Confidence level | Medium |
| Explanation of confidence level | The tiger beetle’s DNA did not show up during the electrophoresis test, but the test proved that the tiger beetle was wolbachia infested. Because the tiger beetle DNA did not register during the test I am not extremely confident in the accuracy of these results. |
| Wolbachia 16S sequence | |
| Arthropod COI sequence |
|
| Summary | The Coleoptera was found to be postive for Wolbachia. |
Sehirus cinctus
Melanoplus bruneri
Pterostichus permundus
Melanoplus Spretus
Centipede- Lithobius forficatus