Sample information |
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| Picture |
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|---|---|
| Location | |
| Collection date | 06/06/2022 |
| Captive / Cultivated? | Wild-caught |
| Group | Pingry School |
| Observations | The arthropod specimen was collected from a grass habitat directly adjacent a forest and was observed nectaring on white clover blossoms. |
| Putative identification | Arthropoda Hexapoda Insecta Diptera Syrphidae Sphecomyia Sphecomyia vittata |
Methods |
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| Extraction kit | DNeasy (Qiagen) |
| DNA extraction location | Abdomen |
| Single or Duplex PCR | Single Reaction |
| Gel electrophoresis system | Standard electrophoresis system |
| Buffer | TAE |
| DNA stain | SYBR Safe |
| Gel images |
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| Protocol notes | The Gel Imaging System was programmed incorrectly, resulting in an image of inappropriately high contrast. This experiment was conducted without controls. |
Results |
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| Wolbachia presence | Yes |
| Confidence level | High |
| Explanation of confidence level | Sequencing data and gel electrophoresis results reveal the presence of Wolbachia 16S in the arthropod specimen. |
| Wolbachia 16S sequence | Download FASTA
Download AB1
GCTGTCGTCAGCTCGTGTCGTGAGATGTTGGGTTAAGTCCCGCAACGAGCGCAACCCTCATCCTTAGTTACCATCAGGTAATGCTGGGGACTTTAAGGAAACTGCCAGTGATAAACTGGAGGAAGGTGGGGATGATGTCAAGTCATCATGGCCCTTATGGAGTGGGCTACACACGTGCTACAATGGTGGCTACAATGGGCTGCAAAGTCGCGAGGCTAAGCTAATCCCTTAAAAGCCATCTCAGTTCGGATTGTACTCTGCAACTCGAGTGCATGAAGTTGGAATCGCTAGTAATCGTGGATCAGCACGCCACGGTGAATACGTTCTCGGGTCTTGTACACACTGCCCGTCACGCCATGGGAATTGGT
BLAST at The Wolbachia Project BLAST at NCBI
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| Arthropod COI sequence | Download FASTA
Download AB1
CGGACTTGAGCTGGATAGTAGGAACATCCTTAAGAATTTTAATTCGTGCAGAATTAGGCCATCCTGGAGCATTAATTGGAGATGACCAAATTTATAATGTTATTGTTACTGCTCATGCTTTTGTTATAATTTTTTTTATAGTTATACCAATTATAATTGGAGGATTTGGAAATTGATTAGTTCCTTTAATATTAGGAGCCCCAGATATAGCCTTTCCTCGAATAAATAATATAAGTTTTTGATTACTACCCCCTTCTTTAACTTTATTATTAGTAAGAAGTATAGTAGAAAATGGAGCTGGAACGGGATGAACTGTTTATCCTCCTCTTTCTGCAAGAATTGCCCATGGAGGTGCTTCTGTTGATTTAGCAATTTTTTCTCTACATTTAGCAGGAATATCTTCTATTTTAGGAGCAGTAAATTTTATTACTACTGTTATTAATATACGGTCTACTGGAATTTCATATGATCGAATACCATTATTTGTATGATCAGTAGTAATTACAGCCCTATTACTTTTACTATCATTACCTGTATTAGCGGGAGCAATTACAATACTTTTAACTGATCGAAATTTAAATACCTCTTTTTTTGATCCAGCAGGTGGGGGAGATCCTATTCTTTACCAACATTTATTTTGA
BLAST at The Wolbachia Project BLAST at NCBI
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| Summary | The Sphecomyia vittata was found to be postive for Wolbachia. |
Water Boatman