Sphecomyia vittata

June 9, 2022

Sample information
Picture
Photos by: Luke E.
Location
Collection date 06/06/2022
Captive / Cultivated? Wild-caught
Group Pingry School
Observations

The arthropod specimen was collected from a grass habitat directly adjacent a forest and was observed nectaring on white clover blossoms.
Putative identification Arthropoda Hexapoda Insecta Diptera Syrphidae Sphecomyia Sphecomyia vittata

Methods
Extraction kit DNeasy (Qiagen)
DNA extraction location Abdomen
Single or Duplex PCR Single Reaction
Gel electrophoresis system Standard electrophoresis system
Buffer TAE
DNA stain SYBR Safe
Gel images
Protocol notes

The Gel Imaging System was programmed incorrectly, resulting in an image of inappropriately high contrast. This experiment was conducted without controls.

Results
Wolbachia presence Yes
Confidence level High
Explanation of confidence level

Sequencing data and gel electrophoresis results reveal the presence of Wolbachia 16S in the arthropod specimen.
Wolbachia 16S sequence Download FASTA    Download AB1
GCTGTCGTCAGCTCGTGTCGTGAGATGTTGGGTTAAGTCCCGCAACGAGCGCAACCCTCATCCTTAGTTACCATCAGGTAATGCTGGGGACTTTAAGGAAACTGCCAGTGATAAACTGGAGGAAGGTGGGGATGATGTCAAGTCATCATGGCCCTTATGGAGTGGGCTACACACGTGCTACAATGGTGGCTACAATGGGCTGCAAAGTCGCGAGGCTAAGCTAATCCCTTAAAAGCCATCTCAGTTCGGATTGTACTCTGCAACTCGAGTGCATGAAGTTGGAATCGCTAGTAATCGTGGATCAGCACGCCACGGTGAATACGTTCTCGGGTCTTGTACACACTGCCCGTCACGCCATGGGAATTGGT
BLAST at The Wolbachia Project   BLAST at NCBI
Arthropod COI sequence Download FASTA    Download AB1
CGGACTTGAGCTGGATAGTAGGAACATCCTTAAGAATTTTAATTCGTGCAGAATTAGGCCATCCTGGAGCATTAATTGGAGATGACCAAATTTATAATGTTATTGTTACTGCTCATGCTTTTGTTATAATTTTTTTTATAGTTATACCAATTATAATTGGAGGATTTGGAAATTGATTAGTTCCTTTAATATTAGGAGCCCCAGATATAGCCTTTCCTCGAATAAATAATATAAGTTTTTGATTACTACCCCCTTCTTTAACTTTATTATTAGTAAGAAGTATAGTAGAAAATGGAGCTGGAACGGGATGAACTGTTTATCCTCCTCTTTCTGCAAGAATTGCCCATGGAGGTGCTTCTGTTGATTTAGCAATTTTTTCTCTACATTTAGCAGGAATATCTTCTATTTTAGGAGCAGTAAATTTTATTACTACTGTTATTAATATACGGTCTACTGGAATTTCATATGATCGAATACCATTATTTGTATGATCAGTAGTAATTACAGCCCTATTACTTTTACTATCATTACCTGTATTAGCGGGAGCAATTACAATACTTTTAACTGATCGAAATTTAAATACCTCTTTTTTTGATCCAGCAGGTGGGGGAGATCCTATTCTTTACCAACATTTATTTTGA
BLAST at The Wolbachia Project   BLAST at NCBI
Summary The Sphecomyia vittata was found to be postive for Wolbachia.
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