Sacrophaga sexpunctata

June 9, 2022

Sample information
Picture
Photos by: Nikita K.
Location
Collection date 06/06/2022
Captive / Cultivated? Wild-caught
Group Pingry School
Observations

Has 6 legs

Dark Color Body

Red Eyes

2 Wings

Caught in the woods bye the lunch tables
Putative identification Arthropoda Hexapoda Insecta Diptera Sarcophagidae Sarcophaga Sarcophaga sexpunctata

Methods
Extraction kit DNeasy (Qiagen)
DNA extraction location Abdomen
Single or Duplex PCR Single Reaction
Gel electrophoresis system Standard electrophoresis system
Buffer TAE
DNA stain SYBR Safe
Gel images
Protocol notes

Ran without controls

Results
Wolbachia presence Yes
Confidence level Medium
Explanation of confidence level

The Blast N results gave me a Query Cover of 94%. Blast X Query Cover was around 90%.
Wolbachia 16S sequence Download FASTA    Download AB1
GTGTTGCATGGCTGTCGTCAGCTCGTGTCGTGAGATGTTGGGTTAAGTCCCGCAACGAGCGCAACCCTCATCCTTAGTTACCATCAGGTAATGCTGGGGACTTTAAGGAAACTGCCAGTGATAAACTGGAGGAAGGTGGGGATGATGTCAAGTCATCATGGCCCTTATGGAGTGGGCTACACACGTGCTACAATGGTGGCTACAATGGGCTGCAAAGTCGCGAGGCTAAGCTAATCCCTTAAAAGCCATCTCAGTTCGGATTGTACTCTGCAACTCGAGTGCATGAAGTTGGAATCGCTAGTAATCGTGGATCAGCACGCCACGGTGAATACGTTCTCGGGTCTTGTACACACTGCCCGTCACGCCATGGGAATTG
BLAST at The Wolbachia Project   BLAST at NCBI
Arthropod COI sequence Download FASTA    Download AB1
GATGACCAAATTTATAATGTAATTGTAACAGCTCATGCTTTTATTATAATTTTTTTTATAGTTATACCAATTATAATTGGAGGATTTGGTAATTGATTAGTTCCTCTTATACTAGGAGCTCCTGATATAGCTTTCCCTCGAATAAACAATATAAGATTTTGACTTCTTCCTCCTGCACTAACTTTACTATTAATAAATACATTAATAGAAAATGGAGCTGGAACAGGATGAACTGTTTATCCTCCCTTATCATCTAATATTGCTCATAGAGGACCTTCTGTAGATTTAACTATTTTTTCTCTTCATTTACTGGAATTTCTTCAATTTTAGGAGCTGTAAATTTTATTACAACAACTATTAATATGCGTTCAACAGGTATTACTTTTGATCGAATACCTTTATTTGTTTGATCAGTATTATTACAGTATTCTTTTATTACTTTCTTTACCAGTTCTTGCGGACAATTACTATATTATTAACTGATCGAAATATAAATACTTCCTTTTTTGACCCACAGGAGGAGGA
BLAST at The Wolbachia Project   BLAST at NCBI
Summary The Sarcophaga sexpunctata was found to be postive for Wolbachia.
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