Tachypodoiulus niger BK02

June 14, 2022

Sample information
Picture
Photos by: Benoit Kunz
Location
Collection date 04/30/2021
Captive / Cultivated? Wild-caught
Group KantiWil
Observations
Putative identification Arthropoda Myriapoda Diplopoda Julida Julidae Tachypodoiulus Tachypodoiulus niger

Methods
Extraction kit DNeasy (Qiagen)
DNA extraction location Partial abdomen
Single or Duplex PCR Duplex Reaction
Gel electrophoresis system Standard electrophoresis system
Buffer TAE
DNA stain SYBR Safe
Gel images
Protocol notes

We cut the organism lengthwise in 4 parts from head to end and take the third quarter

Results
Wolbachia presence Unknown
Confidence level Medium
Explanation of confidence level

No bands of amplification are visible

Line 02: no CO1-Band, no W-Band

in the same series – Drosophila positive control Vanderbilt worked well, see BK26
Wolbachia 16S sequence
Arthropod COI sequence
Summary
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