Sample information |
|
| Picture |
|
|---|---|
| Location | |
| Collection date | 04/30/2021 |
| Captive / Cultivated? | Captive / Cultivated |
| Group | KantiWil |
| Observations | |
| Putative identification | Arthropoda Hexapoda Insecta Diptera Muscidae Musca Musca domestica |
Methods |
|
| Extraction kit | DNeasy (Qiagen) |
| DNA extraction location | Abdomen |
| Single or Duplex PCR | Duplex Reaction |
| Gel electrophoresis system | Standard electrophoresis system |
| Buffer | TAE |
| DNA stain | SYBR Safe |
| Gel images |
|
| Protocol notes | |
Results |
|
| Wolbachia presence | No |
| Confidence level | High |
| Explanation of confidence level | CO1-Band very clear visible, no W-Band |
| Wolbachia 16S sequence | |
| Arthropod COI sequence | Download FASTA
Download AB1
CCTCTCTAAGAATTTTAATTCGAGCAGAACTAGGACATCCTGGAGCATTAATTGGCGATGATCAAATTTATAATGTAATTGTAACAGCTCATGCCTTTGTTATAATTTTTTTTATAGTAATACCTATTATAATTGGAGGATTTGGAAATTGACTTGTCCCTTTAATACTAGGAGCACCTGATATAGCATTTCCTCGAATGAATAATATAAGTTTTTGACTTTTACCTCCTGCACTTACACTACTATTGGTGAGCAGTATAGTGGAAAACGGAGCTGGGACAGGATGAACTGTTTACCCTCCTCTATCTTCTAATATTGCTCATGGAGGAGCTTCTGTTGATTTAGCTATCTTTTCCCTTCATTTAGCAGGAATTTCTTCAATTTTAGGAGCAGTTAATTTTATTACAACTGTTATTAATATGCGATCTACAGGAATTACATTTGACCGAATACCTTTATTTGTTTGATCTGTAGTAATTACAGCATTATTACTCCTTTTGTCCTTACCTGTATAGCAGGAGCTATTACTATATTATTAACAGATCGAAATTTAAATACTTCATTTTTTGACCCTGCAGGAGGAGGTGA
BLAST at The Wolbachia Project BLAST at NCBI
|
| Summary | The Musca domestica was found to be negative for Wolbachia. |
Millipede
Cylindroiulus caeruleocinctus
Biting Midge
Eastern Red Centipede
Specimen_3