Agriotes sp. (Larvae) BK03

June 14, 2022

Sample information
Picture
Photos by: Benoit Kunz
Location
Collection date 04/30/2021
Captive / Cultivated? Wild-caught
Group KantiWil
Observations
Putative identification Arthropoda Hexapoda Insecta Coleoptera Elateridae Agriotes

Methods
Extraction kit DNeasy (Qiagen)
DNA extraction location Other
Single or Duplex PCR Duplex Reaction
Gel electrophoresis system Standard electrophoresis system
Buffer TAE
DNA stain SYBR Safe
Gel images
Protocol notes

We cut the organism lengthwise in 4 parts from head to end and take the third quarter

Results
Wolbachia presence No
Confidence level Medium
Explanation of confidence level

CO1-Band is visible, no W-Band is visible
Wolbachia 16S sequence
Arthropod COI sequence
Summary The Agriotes was found to be negative for Wolbachia.
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