Sample information |
|
| Picture |
|
|---|---|
| Location | |
| Collection date | 04/30/2021 |
| Captive / Cultivated? | Wild-caught |
| Group | KantiWil |
| Observations | |
| Putative identification | Arthropoda Hexapoda Insecta Coleoptera Elateridae Agriotes |
Methods |
|
| Extraction kit | DNeasy (Qiagen) |
| DNA extraction location | Other |
| Single or Duplex PCR | Duplex Reaction |
| Gel electrophoresis system | Standard electrophoresis system |
| Buffer | TAE |
| DNA stain | SYBR Safe |
| Gel images |
|
| Protocol notes | We cut the organism lengthwise in 4 parts from head to end and take the third quarter |
Results |
|
| Wolbachia presence | No |
| Confidence level | Medium |
| Explanation of confidence level | CO1-Band is visible, no W-Band is visible |
| Wolbachia 16S sequence | |
| Arthropod COI sequence |
|
| Summary | The Agriotes was found to be negative for Wolbachia. |
Water Boatman