Lithobius forficatus BK22

Sample information

Picture
Photos by: Benoit K.
Location
Collection date 04/30/2021
Captive / Cultivated? Wild-caught
Group KantiWil
Observations
Putative identification Arthropoda Myriapoda Chilopoda Lithobiomorpha Lithobiidae Lithobius Lithobius forficatus

Methods

Extraction kit DNeasy (Qiagen)
DNA extraction location Rear half
Single or Duplex PCR Duplex Reaction
Gel electrophoresis system Standard electrophoresis system
Buffer TAE
DNA stain SYBR Safe
Gel images
Protocol notes

Results

Wolbachia presence No
Confidence level High
Explanation of confidence level

Line 22: Co1-Band is clearly visible, no W-Band

Wolbachia 16S sequence
Arthropod COI sequence Download FASTA    Download AB1
ATTAAGCCAACCAGGGAGACTTATTGGAGATGACCAAATTWATAATGTAATCGTAACAGCCCACGCATTCATTATAATCTTCTTTATAGTAATACCTATCATAATTGGAGGATTCGGTAATTGATTGGTGCCCTTAATACTAGGGGCACCAGACATAGCTTTCCCACGATTAAACAATATAAGATTTTGACTATTGCCGCCCTCACTAACGTTACTTCTATCCTCAGCTGCCGTAGAAAGAGGGGCAGGAACAGGATGAACAGTCTACCCCCCCTTGTCTTCTAATATCTCCCATAGTGGAGCATCAGTTGATATAACAATTTTCTCTCTTCATTTAGCAGGTGCATCCTCAATTCTAGGGGCAATCAATTTCATTTCAACAATCATTAACATACGATCAAGAGGAATAACATTTGAGCGAGTACCATTATTCGTATGATCTGTAAAAATCACAGTAATTCTTCTATTATTATCTTTGCCCGTATTAGCAGGAGCAATCACAATACTATTAACAGATCGAAACTTAAATACAAGCTTCTTTGACCCAACAGGAGGAGGAGACCCCATCCTATATCAACATCTATTCTGATTTTTGTMAGAAAAAAAGAGAGAAAAAAAAAAAAAA
BLAST at The Wolbachia Project   BLAST at NCBI
Summary The Lithobius forficatus was found to be negative for Wolbachia.
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