Phosphuga atrata BK23

June 16, 2022

Sample information
Picture
Photos by: Benoit K.
Location
Collection date 04/30/2021
Captive / Cultivated? Wild-caught
Group KantiWil
Observations
Putative identification Arthropoda Hexapoda Insecta Coleoptera Silphidae Phosphuga Phosphuga atrata

Methods
Extraction kit DNeasy (Qiagen)
DNA extraction location Gonads dissected
Single or Duplex PCR Duplex Reaction
Gel electrophoresis system Standard electrophoresis system
Buffer TAE
DNA stain SYBR Safe
Gel images
Protocol notes

Results
Wolbachia presence Unknown
Confidence level Medium
Explanation of confidence level

Line 23: no CO1-Band, no W-Band

in the same series – Drosophila positive control Vanderbilt worked well, see line 26
Wolbachia 16S sequence
Arthropod COI sequence
Summary
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