Sample information |
|
| Picture |
|
|---|---|
| Location | |
| Collection date | 04/30/2021 |
| Captive / Cultivated? | Wild-caught |
| Group | KantiWil |
| Observations | |
| Putative identification | Arthropoda Hexapoda Insecta Coleoptera Cerambycidae Hylotrupes Hylotrupes bajulus |
Methods |
|
| Extraction kit | DNeasy (Qiagen) |
| DNA extraction location | Larva |
| Single or Duplex PCR | Duplex Reaction |
| Gel electrophoresis system | Standard electrophoresis system |
| Buffer | TAE |
| DNA stain | SYBR Safe |
| Gel images |
|
| Protocol notes | We cut the organism lengthwise in 4 parts from head to end and take the third quarter |
Results |
|
| Wolbachia presence | No |
| Confidence level | High |
| Explanation of confidence level | Line 25: Co1-Band is clearly visible, no W-Band |
| Wolbachia 16S sequence | |
| Arthropod COI sequence | Download FASTA
Download AB1
CAGGAATAGTTGGRACATCCTTAAGATTGTTAATTCGAACAGAACTAGGAAATCCAGGATCTTTAATTGGAGATGATCAAATCTATAATGTTATTGTTACTGCCCATGCTTTTATTATAATTTTTTTCATAGTTATACCAATTATAATTGGAGGATTTGGAAACTGATTAGTTCCCCTAATATTGGGGGCTCCCGATATAGCTTTTCCTCGAATAAATAATATAAGATTTTGACTTTTACCTCCTTCTTTAACTTTATTAATCATAAGAAGAGTGGTAGAAAATGGGGCTGGTACCGGATGAACCGTCTATCCCCCTCTTTCTTCAAATATTGCTCATAGAGGATCTTCAGTTGATTTAGCAATTTTTAGGTTACATTTAGCAGGTATCTCTTCAATTCTTGGTGCAGTAAATTTTATCACTACAGTAATTAATATACGACCAATTGGAATAACTCCTGATCGTGTTCCTTTATTTGTTTGGGCAGTAGTAATTACTACAATTCTTCTTCTTCTTTCATTACCAGTTTTAGCTGGAGCAATTACTATACTTTTAACAGATCGAAATTTAAATACTTCATTTTTTGATCCTGCAGGAGGTGGTGATCCTATTCTTTATCAACATTTATTTTGATTTTTTGTCACAC
BLAST at The Wolbachia Project BLAST at NCBI
|
| Summary | The Hylotrupes bajulus was found to be negative for Wolbachia. |
Vespidae Maculifrons
Tenebrio molitor
Wasp-Hymenoptera sp
Pollenia pediculata,
Phyciodes tharos