Hylotrupes bajulus BK25

June 16, 2022

Sample information
Picture
Photos by: Benoit K.
Location
Collection date 04/30/2021
Captive / Cultivated? Wild-caught
Group KantiWil
Observations
Putative identification Arthropoda Hexapoda Insecta Coleoptera Cerambycidae Hylotrupes Hylotrupes bajulus

Methods
Extraction kit DNeasy (Qiagen)
DNA extraction location Larva
Single or Duplex PCR Duplex Reaction
Gel electrophoresis system Standard electrophoresis system
Buffer TAE
DNA stain SYBR Safe
Gel images
Protocol notes

We cut the organism lengthwise in 4 parts from head to end and take the third quarter

Results
Wolbachia presence No
Confidence level High
Explanation of confidence level

Line 25: Co1-Band is clearly visible, no W-Band
Wolbachia 16S sequence
Arthropod COI sequence Download FASTA    Download AB1
CAGGAATAGTTGGRACATCCTTAAGATTGTTAATTCGAACAGAACTAGGAAATCCAGGATCTTTAATTGGAGATGATCAAATCTATAATGTTATTGTTACTGCCCATGCTTTTATTATAATTTTTTTCATAGTTATACCAATTATAATTGGAGGATTTGGAAACTGATTAGTTCCCCTAATATTGGGGGCTCCCGATATAGCTTTTCCTCGAATAAATAATATAAGATTTTGACTTTTACCTCCTTCTTTAACTTTATTAATCATAAGAAGAGTGGTAGAAAATGGGGCTGGTACCGGATGAACCGTCTATCCCCCTCTTTCTTCAAATATTGCTCATAGAGGATCTTCAGTTGATTTAGCAATTTTTAGGTTACATTTAGCAGGTATCTCTTCAATTCTTGGTGCAGTAAATTTTATCACTACAGTAATTAATATACGACCAATTGGAATAACTCCTGATCGTGTTCCTTTATTTGTTTGGGCAGTAGTAATTACTACAATTCTTCTTCTTCTTTCATTACCAGTTTTAGCTGGAGCAATTACTATACTTTTAACAGATCGAAATTTAAATACTTCATTTTTTGATCCTGCAGGAGGTGGTGATCCTATTCTTTATCAACATTTATTTTGATTTTTTGTCACAC
BLAST at The Wolbachia Project   BLAST at NCBI
Summary The Hylotrupes bajulus was found to be negative for Wolbachia.
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