|Captive / Cultivated?||Captive / Cultivated|
|Group||South Plainfield High School|
Female larva captured by the Middlesex Mosquito commission, grown to adulthood, and donated to our class.
|Putative identification||Hexapoda Insecta Diptera Culicidae Culex|
|Extraction kit||Monarch DNA extraction (NEB)|
|DNA extraction location||Abdomen|
|Single or Duplex PCR||Single Reaction|
|Gel electrophoresis system||MiniOne|
|Explanation of confidence level||
No band was present at the 500 level which shows that Wolbachia was not present in the insect.
|Wolbachia 16S sequence||
|Arthropod COI sequence||
|Summary||The Culex was found to be negative for Wolbachia.|