Mosquito – Lorenzo Fuscoletti

October 24, 2022

Sample information
Picture
Photos by: Lorenzo F
Location
Collection date 09/20/2022
Captive / Cultivated? Captive / Cultivated
Group South Plainfield High School
Observations

Female mosquito caputerd and grown into adults by the Middlesex County Mosquito Commission and donated to our class.
Putative identification Arthropoda Hexapoda Insecta Diptera Culicidae Culex

Methods
Extraction kit
DNA extraction location
Single or Duplex PCR Single Reaction
Gel electrophoresis system
Buffer
DNA stain
Gel images
Protocol notes

Modification P2 for ATL and N3 for AL

Results
Wolbachia presence
Confidence level
Explanation of confidence level

Since our DNA ran off the gel, we had no results to see if they had wolbachia. Testing in our gel for wolbachia, my mosquito DNA had a band showing wolbachia.
Wolbachia 16S sequence
Arthropod COI sequence
Summary
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