Sample information
Picture	Photos by: Nivash B.
Location
Collection date	09/20/2022
Captive / Cultivated?	Captive / Cultivated
Group	South Plainfield High School
Observations	Female, Larvae captured by Middlesex Mosquito Commission, grown to adults
Putative identification	Arthropoda Hexapoda Insecta Diptera Culicidae Culex
Methods
Extraction kit	Qiagen Blood and Tissue kit
DNA extraction location	Abdomen
Single or Duplex PCR	Single Reaction
Gel electrophoresis system	MiniOne
Buffer	TAE
DNA stain	Other
Gel images
Protocol notes
Results
Wolbachia presence	No
Confidence level	High
Explanation of confidence level
Wolbachia 16S sequence
Arthropod COI sequence
Summary	The Culex was found to be negative for Wolbachia.