Mosquito – Ashlynn Lehman

October 24, 2022

Sample information
Picture
Photos by: Ashlynn L
Location No map location data available.
Collection date 09/20/2022
Captive / Cultivated? Captive / Cultivated
Group South Plainfield High School
Observations

Female larvae were captured in Cheesequake State Park (Old Bridge, NJ) and raised by the Middlesex Mosquito Commission, then donated to our class.

The mosquito DNA tested negative for Wolbachia, as there is no band present at the 500 mark.  However, there are no bands present for Ashlynn’s sample (the third column) or Diya Patel’s (the fifth column), so it is possible that the results were not accurate as the mosquito DNA did not show up when it was supposed to.
Putative identification Arthropoda Hexapoda Insecta Diptera Culicidae Culex

Methods
Extraction kit Qiagen Blood and Tissue Kit - With Modifications - P2 for ATL and N3 for AL
DNA extraction location Abdomen
Single or Duplex PCR Single Reaction
Gel electrophoresis system MiniOne
Buffer TAE
DNA stain Other
Gel images
Protocol notes

Modification of P2 for ATL and N3 for AL

Results
Wolbachia presence No
Confidence level Medium
Explanation of confidence level

This is only our second time running gels, so there is a possibility that our results are not completely accurate as we have not yet mastered the procedure.  However, the ladders were present, which increases our confidence in our results.
Wolbachia 16S sequence
Arthropod COI sequence
Summary The Culex was found to be negative for Wolbachia.
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