Sample information |
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| Picture |
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|---|---|
| Location | |
| Collection date | 09/13/2022 |
| Captive / Cultivated? | Wild-caught |
| Group | KantiWil |
| Observations | Electoral path |
| Putative identification | Arthropoda Insecta Hymenoptera Formicidae Lasius Lasius niger |
Methods |
|
| Extraction kit | Instagene Matrix |
| DNA extraction location | Whole arthropod |
| Single or Duplex PCR | Duplex Reaction |
| Gel electrophoresis system | Standard electrophoresis system |
| Buffer | TAE |
| DNA stain | SYBR Safe |
| Gel images |
|
| Protocol notes | |
Results |
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| Wolbachia presence | No |
| Confidence level | High |
| Explanation of confidence level | There is clearly only the band of the Insect-DNA visible |
| Wolbachia 16S sequence | Download FASTA
Download AB1
|
| Arthropod COI sequence |
AGCCGGTATAATTGGCTCATCTATAAGAATAATCATTCGACTAGAATTAGGTTCTTCTAATTCATTAATTAATAATGATC AAATTTATAATTCTATAGTTACAAGACATGCATTCGTTATAATTTTCTTTATAGTTATGCCTTTCATAATTGGAGGATTT GGAAATTTTCTCGTACCTTTAATATTAGGATCACCTGATATAGCATACCCCCGTATAAATAATATAAGATTTTGACTTTT ACCCCCTTCAATTTCTCTCCTTCTTTTAAGAAATTTTATTAATGACGGAGTAGGAACAGGATGAACTGTTTATCCTCCAT TAGCTTCTAATATTTTTCATAATGGTCCCTCAGTTGATTTAACTATTTTTTCTCTTCATATTGCTGGAATATCTTCTATT CTAGGAGCTATCAATTTTATTTCAACTATTATAAATATACATCATAAAAATTTTTCTATCGATAAAATTCCCTTACTTGT ATGATCAATTTTAATCACTGCAATTTTATTACTTTTATCCCTCCCAGTTCTTGCAGGAGCTATCACTATACTTCTAACTG ACCGTAACCTTAATACTTCATTTTTTGACCCATCTGGTGGTGGTGATCCTATTTTATACCAACATCTCTTCTGATTTTTT GGCCAC
BLAST at The Wolbachia Project BLAST at NCBI
|
| Summary | The Lasius niger was found to be negative for Wolbachia. |
Common Eastern Bumble Bee (Bombus impatiens)
American Bird
Spotted crane fly
Wolbachia data
Meadow Katydid