Sample information |
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| Picture |
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|---|---|
| Location | |
| Collection date | 09/13/2022 |
| Captive / Cultivated? | Wild-caught |
| Group | St. Albans School |
| Observations | Respectably sized cricket, captured alive in the summer. |
| Putative identification | Arthropoda Hexapoda Insecta Orthoptera Gryllidae Acheta Acheta domesticus |
Methods |
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| Extraction kit | DNeasy (Qiagen) |
| DNA extraction location | Abdomen |
| Single or Duplex PCR | Single Reaction |
| Gel electrophoresis system | MiniPCR |
| Buffer | TBE |
| DNA stain | GelGreen |
| Gel images |
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| Protocol notes | |
Results |
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| Wolbachia presence | No |
| Confidence level | High |
| Explanation of confidence level | All our controls worked as expected and the results matched the gel electrophoresis. |
| Wolbachia 16S sequence | |
| Arthropod COI sequence | Download FASTA
Download AB1
CTCTTTAAGTATCTTAATTCNAACGGAACTAGGACAACCANGNTATTTAATTGGAGATGATCAAACATATAATGTTATCGTAACTGCACATGCATTTGTCATAATTTTTTTCATGGNNATACCAATTATNNTTGNNGGATTCGGAAATTGATTAGTACCCCTAATATTAGGTGCACCCGATATAGCCTTTCCTCGAATAAACNNNATAAGATTTTGANTTTTACCACCCTCACTAACCCTTTTATTAACCAGAAGAATAGTCGAAAATGGTGCAGGGACAGGATGAACAGTTTATCCACCTTTATCAACAGGAATCGCCCACGCCGGAGCATCTGTTGATTTAGCCATTTTTTCATTACACTTAGCTGGAATTTCATCAATTCTGGGAGCCGTTAATTTCATTACAACTATAATCAATATACGAGCACCTGGGATGTCATTAGATCAAACCCCATTATTTGTATGAGCTGTNNNNNNCACTGCCCTCCTCCTATTATTATCTTTACCTGTTCTTGCGGGTGCAATCACAATATTACTAACAGATCGAAATCTGAATACATCATTTTTTGACCCCGCAGGAGGGGGTGATCCCATCTTATATCAACACTTATTTT
BLAST at The Wolbachia Project BLAST at NCBI
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| Summary | The Acheta domesticus was found to be negative for Wolbachia. |
Millipede
Cylindroiulus caeruleocinctus
Biting Midge
Eastern Red Centipede
Specimen_3