Sample information |
|
| Picture |
|
|---|---|
| Location | |
| Collection date | 09/14/2022 |
| Captive / Cultivated? | Wild-caught |
| Group | St. Albans School |
| Observations | active, collected in dirt in the afternoon |
| Putative identification | Arthropoda Hexapoda Insecta Lepidoptera |
Methods |
|
| Extraction kit | DNeasy (Qiagen) |
| DNA extraction location | Abdomen |
| Single or Duplex PCR | Single Reaction |
| Gel electrophoresis system | MiniPCR |
| Buffer | TBE |
| DNA stain | GelGreen |
| Gel images |
|
| Protocol notes | |
Results |
|
| Wolbachia presence | No |
| Confidence level | High |
| Explanation of confidence level | All controls worked as expected and there were no issues with protocol. Gels and controls were visible as bands. |
| Wolbachia 16S sequence | Download FASTA
Download AB1
|
| Arthropod COI sequence |
NNNNNNANGANCTTCCTNNNNNNNNCTGANTCNANCNNAATTANGNANTCCNGNNNCNNNNNTTGGANATGATNNNNNNNNTNNTACTATTGNNACNGCTCATGCTTTTATTATAATTTTTTTTATANNTATACNNNNTNTNATTGGAGGATTTGGAAATTGATTANNACCTTTAATACTANGANCTCCTGATNTANCTTTCCCCCNAATAAATAATATNNNNNNNTGAATATTACCCCCTTCATTAATATTATTAATTTCAAGAANAATTGTANAAAATGGTGCAGGAACAGGTTGAACAGTTTACCCCCCTTTATCTTCTAATATTGCTCATCNNGGATCTTCTGTTGATTTANCAATTTTTTCTCTTCATTTANCTGGAATCTCTTCAATTTTAGGAGCTATTAATTTTATTACAACAATCATTAATATACGAATTAAAAATTTATCATTTGACCAAATACCTTTATTTGTTTGATCTGNANGAATTACAGCTTTATTGTTACTCTTATCTTTACCTGTTTTAGCANGANCTANTACTATACTACTTACNGANCGAANNTTAAATACTTNTTTTTTTNANCNNGCTGNANNANGGGNNCNNNNTNNATACCAACATTTATTTTGATTTTTTGGTCACCNNNNNNNTTNNAN
BLAST at The Wolbachia Project BLAST at NCBI
|
| Summary | The Lepidoptera was found to be negative for Wolbachia. |
Water Boatman