Sample information |
|
| Picture |
|
|---|---|
| Location | |
| Collection date | 08/22/2022 |
| Captive / Cultivated? | Wild-caught |
| Group | Kantonsschule Zuercher Unterland |
| Observations |
|
| Putative identification | Arthropoda Hexapoda Insecta Blattodea Blaberoidea Ectobiidae Ectobius Ectobius vittiventris |
Methods |
|
| Extraction kit | Instagene Matrix |
| DNA extraction location | Rear half |
| Single or Duplex PCR | Single Reaction |
| Gel electrophoresis system | MiniOne |
| Buffer | TBE |
| DNA stain | Fast Blast |
| Gel images |
|
| Protocol notes | The process overall was probaly well done. We had to do it step by step and did not really face any difficulties. |
Results |
|
| Wolbachia presence | No |
| Confidence level | Medium |
| Explanation of confidence level | We did it in school. Therefore we are not sure about how exact and sterile it was done. |
| Wolbachia 16S sequence | |
| Arthropod COI sequence |
|
| Summary | The Ectobius vittiventris was found to be negative for Wolbachia. |
Vespidae Maculifrons
Tenebrio molitor
Wasp-Hymenoptera sp
Pollenia pediculata,
Phyciodes tharos