Sample information |
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| Picture |
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|---|---|
| Location | |
| Collection date | 09/14/2022 |
| Captive / Cultivated? | Wild-caught |
| Group | St. Albans School |
| Observations | Found sitting on top of the grill cover in my backyard. It was about 8 pm and around 65 degrees Fahrenheit. |
| Putative identification | Arthropoda Hexapoda Insecta Diptera |
Methods |
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| Extraction kit | DNeasy (Qiagen) |
| DNA extraction location | Whole arthropod |
| Single or Duplex PCR | Single Reaction |
| Gel electrophoresis system | MiniPCR |
| Buffer | TBE |
| DNA stain | GelGreen |
| Gel images |
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| Protocol notes | |
Results |
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| Wolbachia presence | No |
| Confidence level | High |
| Explanation of confidence level | All of the controls worked as expected, and I was not surprised by any of the bands on the gel. Therefore, I am confident that my arthropod is not infected with Wolbachia. |
| Wolbachia 16S sequence | |
| Arthropod COI sequence |
GANCAGGANNAGTAGGAACTTCTCTTAGAATTCTTATTCGAGCAGAATTAGGTCACCCTGGAGCATTAATTGGTGATGATCAAATTTATAATGTTATTGTAACTGCCCATGCCTTTATCATAATTTTTTTTATAGTAATACCTATTATAATTGGGGGATTTGGAAATTGATTAGTTCCATTAATATTAGGAGCTCCAGATATAGCTTTTCCTCGAATAAATAATATAAGATTTTGATTATTACCTCCTTCATTAACTCTTTTATTAGCAAGAAGAATAGTAGATAATGGAGCAGGAACAGGTTGAACAGTTTATCCCCCTTTATCTTCAGGAATTGCTCATAGAGGAGCTTCTGTTGATTTAGCTATTTTTTCTTTACATTTAGCCGGAGTATCTTCTATTTTAGGGGCTGTAAATTTTATTACAACTGTTATTAATATACGAGCTACAGGAATTTCATTTGACCGAATACCTTNATTTNTTTNATCTGTAGTTATTACAGCTGTACTATTACTATTATCATTACCTGTATTAGCGGGAGCTATTACAATACTATTAACTGATCGAAATTTAAATACTTCTTTTTTTGANCCTGCAGGAGGAGGAGANCCTATTCNTTATC
BLAST at The Wolbachia Project BLAST at NCBI
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| Summary | The Diptera was found to be negative for Wolbachia. |
Water Boatman