Rhipidia

November 2, 2022

Sample information
Picture
Photos by: Kaitlyn B
Location
Collection date 09/14/2022
Captive / Cultivated? Wild-caught
Group St. Albans School
Observations

Found sitting on top of the grill cover in my backyard. It was about 8 pm and around 65 degrees Fahrenheit.
Putative identification Arthropoda Hexapoda Insecta Diptera

Methods
Extraction kit DNeasy (Qiagen)
DNA extraction location Whole arthropod
Single or Duplex PCR Single Reaction
Gel electrophoresis system MiniPCR
Buffer TBE
DNA stain GelGreen
Gel images
Protocol notes

Results
Wolbachia presence No
Confidence level High
Explanation of confidence level

All of the controls worked as expected, and I was not surprised by any of the bands on the gel. Therefore, I am confident that my arthropod is not infected with Wolbachia.
Wolbachia 16S sequence
Arthropod COI sequence
GANCAGGANNAGTAGGAACTTCTCTTAGAATTCTTATTCGAGCAGAATTAGGTCACCCTGGAGCATTAATTGGTGATGATCAAATTTATAATGTTATTGTAACTGCCCATGCCTTTATCATAATTTTTTTTATAGTAATACCTATTATAATTGGGGGATTTGGAAATTGATTAGTTCCATTAATATTAGGAGCTCCAGATATAGCTTTTCCTCGAATAAATAATATAAGATTTTGATTATTACCTCCTTCATTAACTCTTTTATTAGCAAGAAGAATAGTAGATAATGGAGCAGGAACAGGTTGAACAGTTTATCCCCCTTTATCTTCAGGAATTGCTCATAGAGGAGCTTCTGTTGATTTAGCTATTTTTTCTTTACATTTAGCCGGAGTATCTTCTATTTTAGGGGCTGTAAATTTTATTACAACTGTTATTAATATACGAGCTACAGGAATTTCATTTGACCGAATACCTTNATTTNTTTNATCTGTAGTTATTACAGCTGTACTATTACTATTATCATTACCTGTATTAGCGGGAGCTATTACAATACTATTAACTGATCGAAATTTAAATACTTCTTTTTTTGANCCTGCAGGAGGAGGAGANCCTATTCNTTATC
BLAST at The Wolbachia Project   BLAST at NCBI
Summary The Diptera was found to be negative for Wolbachia.
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