Schwarzköpfiger Wegerich-Erdfloh

November 10, 2022

Sample information
Picture
Photos by: Anisha H., Victoria S.
Location
Collection date 08/22/2022
Captive / Cultivated? Wild-caught
Group Kantonsschule Zuercher Unterland
Observations

Im hohen Gebüsch am Wegrand des Biotops der KZU gefangen.
Putative identification Arthropoda Hexapoda Insecta Coleoptera Chrysomelidae Longitarsus Longitarsus melanocephalus

Methods
Extraction kit Instagene Matrix
DNA extraction location Partial abdomen
Single or Duplex PCR Single Reaction
Gel electrophoresis system MiniOne
Buffer TBE
DNA stain Other
Gel images
Protocol notes

Gel Stain: Midori 1:20’000

Results
Wolbachia presence No
Confidence level Medium
Explanation of confidence level

Our Quality Values were high, so we think we can trust our results. Also the result of the arthropod was the same image in the internet as ours.
For that reason we chose the medium confidence level, because we have good results but there can always happen a small mistake.
Wolbachia 16S sequence
Arthropod COI sequence Download FASTA    Download AB1
Summary The Longitarsus melanocephalus was found to be negative for Wolbachia.
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