Sample information |
|
| Picture |
|
|---|---|
| Location | |
| Collection date | 09/14/2022 |
| Captive / Cultivated? | Wild-caught |
| Group | St. Albans School |
| Observations | |
| Putative identification | Arthropoda Hexapoda Insecta Hymenoptera |
Methods |
|
| Extraction kit | DNeasy (Qiagen) |
| DNA extraction location | Abdomen |
| Single or Duplex PCR | Single Reaction |
| Gel electrophoresis system | MiniPCR |
| Buffer | TBE |
| DNA stain | GelGreen |
| Gel images |
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| Protocol notes | |
Results |
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| Wolbachia presence | No |
| Confidence level | High |
| Explanation of confidence level | Our controls worked and the bee didn’t have Wolbachia |
| Wolbachia 16S sequence | |
| Arthropod COI sequence | Download FASTA
Download AB1
TAGTGGAACATCAATAAGATNATTAATTCNAATAGAACTTAGACATCCNGGAATATGAATTAATAATGATCAAATTTATTACTCATTAGTTACTAGACATGGCTTTTTATTAATTTTTTTTATAGGTATACCATTTATAATTGGAGGATTCGGAAATTATTTAATTCCTTTAATATTAGGATCACCTGATATAGCTTTTCCCCCAATAAATAATATTAGATTTTGATTATTACCTCCTTCTATTTTTATATTATTATTAAGAACTTTATTTACACCTAATGTAGGAACAGGTTGAACCGCATATCCCCCTTTATCATCTTATTTATTTCCTTCATCACCCTCACCAGATATTGCAATTTTTTCTTTACCTGTAACAGGAATTTCTTCTATTATTGGTTCATTAAATTTTATCGTNNCNATTATATTAATAAAAAATTTTTCATTAAATTATGATCAAATTACTTTATTTTCNTGATCTGTATGTATTACAGTNTTATTATTAATTTTATCATTACCAGTTNNAGCTGGAGCAATTACTATACTTCTTTTTGATCGAAATTTTANTACATCATTTTTTTGATCCAATANNNGGTGGAGATCCAATTTTAT
BLAST at The Wolbachia Project BLAST at NCBI
|
| Summary | The Hymenoptera was found to be negative for Wolbachia. |
Thrip – Phlaeothripidae Leptothrips sp. 21
MACALF24-SB Dermaptera
Thrip – Phlaeothripidae Leptothrips sp. 19