Sample information |
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| Picture |
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|---|---|
| Location | |
| Collection date | 09/14/2022 |
| Captive / Cultivated? | Wild-caught |
| Group | St. Albans School |
| Observations | I captured this arthropod in the courtyard of my school. The weather was warm and sunny, and it was about noon when I caught it. It was climbing on a flower stem, and I caught it before it could fly away. It was the only member of its species that I could find in the courtyard. |
| Putative identification | Arthropoda Hexapoda Insecta Hemiptera |
Methods |
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| Extraction kit | DNeasy (Qiagen) |
| DNA extraction location | Whole arthropod |
| Single or Duplex PCR | Single Reaction |
| Gel electrophoresis system | MiniPCR |
| Buffer | TBE |
| DNA stain | GelGreen |
| Gel images |
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| Protocol notes | |
Results |
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| Wolbachia presence | No |
| Confidence level | High |
| Explanation of confidence level | All of the controls worked properly and there were no issues with the protocol for the experiment. There were no bands on the gel, and for the reasons listed above, I am confident in my result that my arthropod is not infected with Wolbachia. |
| Wolbachia 16S sequence | |
| Arthropod COI sequence |
TATTAATTATCCGCCTCGCATTGGGANNNNNGNGCCGATTCCTTGGTAATGNTCNNCCCNTCATGTAGTTGAANNNGNACATGCATTTGTNATANTTTTCTTTTGGNTNNACCAATCATAATTGGCGGATTCGGCAGNTGATTANTCCCTTTAATAATTGGAGCTCCTGATATAGCCTTCCCACNATTACATAATATAAGATTCTGATTATTACCCCCTTCNTTAACTTTATTATTAATAAGAAGAATAGCAAAATCAAGAGGTGGGAGAGGATGAACAGCCTATCCCCCCTTATCCAGTAATATCTCACATAAAGGAGCATCAGGTGATTTACCTATTTTTTCTTTACTTTTGGTTGGGGTTTCATCAATTTTANGGGCAGTAAATTTTATTTCTACTATTATAAATATACGACCAACAGGTATAACCCCTGAACAAATCCCATTGTTTGTGTGATCANTAGGAATTACTGCACTTCTTCTGTTATTATCCTTACCTGTGTTAGCANGTGCTATTACTATACTATTAACTGACCNAANTTTTAATACCTCTTTTTTTGACCCNTCANGATGANGAG
BLAST at The Wolbachia Project BLAST at NCBI
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| Summary | The Hemiptera was found to be negative for Wolbachia. |
Water Boatman