|Captive / Cultivated?||Wild-caught|
|Group||St. Albans School|
I captured this arthropod in the courtyard of my school. The weather was warm and sunny, and it was about noon when I caught it. It was climbing on a flower stem, and I caught it before it could fly away. It was the only member of its species that I could find in the courtyard.
|Putative identification||Arthropoda Hexapoda Insecta Hemiptera|
|Extraction kit||DNeasy (Qiagen)|
|DNA extraction location||Whole arthropod|
|Single or Duplex PCR||Single Reaction|
|Gel electrophoresis system||MiniPCR|
|Explanation of confidence level||
All of the controls worked properly and there were no issues with the protocol for the experiment. There were no bands on the gel, and for the reasons listed above, I am confident in my result that my arthropod is not infected with Wolbachia.
|Wolbachia 16S sequence||
|Arthropod COI sequence||
TATTAATTATCCGCCTCGCATTGGGANNNNNGNGCCGATTCCTTGGTAATGNTCNNCCCNTCATGTAGTTGAANNNGNACATGCATTTGTNATANTTTTCTTTTGGNTNNACCAATCATAATTGGCGGATTCGGCAGNTGATTANTCCCTTTAATAATTGGAGCTCCTGATATAGCCTTCCCACNATTACATAATATAAGATTCTGATTATTACCCCCTTCNTTAACTTTATTATTAATAAGAAGAATAGCAAAATCAAGAGGTGGGAGAGGATGAACAGCCTATCCCCCCTTATCCAGTAATATCTCACATAAAGGAGCATCAGGTGATTTACCTATTTTTTCTTTACTTTTGGTTGGGGTTTCATCAATTTTANGGGCAGTAAATTTTATTTCTACTATTATAAATATACGACCAACAGGTATAACCCCTGAACAAATCCCATTGTTTGTGTGATCANTAGGAATTACTGCACTTCTTCTGTTATTATCCTTACCTGTGTTAGCANGTGCTATTACTATACTATTAACTGACCNAANTTTTAATACCTCTTTTTTTGACCCNTCANGATGANGAGBLAST at The Wolbachia Project BLAST at NCBI
|Summary||The Hemiptera was found to be negative for Wolbachia.|