Luca’s Fly

November 22, 2022

Sample information
Picture
Photos by: Luca C
Location
Collection date 09/18/2022
Captive / Cultivated? Wild-caught
Group Edmund Burke School
Observations

Anondized greenish blue color, small, resting on leaf when caught, 4 wings, large eyes, six legs. 70 degrees when found. Resting on leaf, near oak tree.

 
Putative identification Arthropoda Hexapoda Insecta Diptera Calliphoridae Lucilia Lucilia sericata

Methods
Extraction kit DNeasy (Qiagen)
DNA extraction location Gonads dissected
Single or Duplex PCR Single Reaction
Gel electrophoresis system MiniOne
Buffer TBE
DNA stain GelGreen
Gel images
Protocol notes

Arthropod Identification Lab Protocol and Post Lab, we also used a 10Kb DNA ladder.

Results
Wolbachia presence No
Confidence level High
Explanation of confidence level

High, due to the controls that we used and the lack of contamination.
Wolbachia 16S sequence Download FASTA    Download AB1
Arthropod COI sequence Download FASTA    Download AB1
Query 1 AATTGGAACTTCTTTAAGAATTCTAATTCGAGCTGAATTAGGACATCCTGGAGCTTTAAT 60 |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| Sbjct 45 AATTGGAACTTCTTTAAGAATTCTAATTCGAGCTGAATTAGGACATCCTGGAGCTTTAAT 104 Query 61 TGGAGATGATCAAATTTATAATGTAATTGTTACAGCTCATGCTTTTATTATAAttttttt 120 |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| Sbjct 105 TGGAGATGATCAAATTTATAATGTAATTGTTACAGCTCATGCTTTTATTATAATTTTTTT 164 Query 121 tATAGTAATGCCAATTATAATTGGAGGATTTGGAAATTGATTAGTTCCATTAATACTAGG 180 |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| Sbjct 165 TATAGTAATGCCAATTATAATTGGAGGATTTGGAAATTGATTAGTTCCATTAATACTAGG 224 Query 181 AGCTCCAGATATAGCATTCCCTCGAATAAATAATATAAGTTTTTGACTTTTACCTCCTGC 240 |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| Sbjct 225 AGCTCCAGATATAGCATTCCCTCGAATAAATAATATAAGTTTTTGACTTTTACCTCCTGC 284 Query 241 ATTAACTTTATTATTAGTTAGTAGTATAGTAGAAAACGGAGCTGGAACAGGATGAACAGT 300 |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| Sbjct 285 ATTAACTTTATTATTAGTTAGTAGTATAGTAGAAAACGGAGCTGGAACAGGATGAACAGT 344 Query 301 TTACCCTCCTCTATCTTCTAATATTGCTCATGGAGGAGCTTCTGTTGATTTAGCTATTTT 360 |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| Sbjct 345 TTACCCTCCTCTATCTTCTAATATTGCTCATGGAGGAGCTTCTGTTGATTTAGCTATTTT 404 Query 361 CTCTCTTCATTTAGCAGGAATTTCTTCAATTTTAGGAGNTGTAAATTTTATTACTACAGT 420 |||||||||||||||||||||||||||||||||||||| ||||||||||||||||||||| Sbjct 405 CTCTCTTCATTTAGCAGGAATTTCTTCAATTTTAGGAGCTGTAAATTTTATTACTACAGT 464 Query 421 TATTAATATACGATCAACAGGAATTACTTTTGATCGAATACCTTTATTTGTTTGATCAGT 480 |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| Sbjct 465 TATTAATATACGATCAACAGGAATTACTTTTGATCGAATACCTTTATTTGTTTGATCAGT 524 Query 481 AGTAATTACAGCTTTATTACTNNTATTATCATTACCAGTATTANCAGGAGCTATTACAAT 540 ||||||||||||||||||||| |||||||||||||||||||| |||||||||||||||| Sbjct 525 AGTAATTACAGCTTTATTACTTTTATTATCATTACCAGTATTAGCAGGAGCTATTACAAT 584 Query 541 ACTTTTAACAGACCGAAATCTTAATACATCATTCTTTGACCCTGCAGGAGG 591 ||||||||||||||||||||||||||||||||||||||||||||||||||| Sbjct 585 ACTTTTAACAGACCGAAATCTTAATACATCATTCTTTGACCCTGCAGGAGG 635
BLAST at The Wolbachia Project   BLAST at NCBI
Summary The Lucilia sericata was found to be negative for Wolbachia.
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