Luca Mantis

November 22, 2022

Sample information
Picture
Photos by: Luca C
Location
Collection date 09/15/2022
Captive / Cultivated? Wild-caught
Group Edmund Burke School
Observations

Caught during summer, all other info n/a, as caught by different person.
Putative identification Arthropoda Hexapoda Insecta Mantodea Mantidae Stagmomantis

Methods
Extraction kit DNeasy (Qiagen)
DNA extraction location Gonads dissected
Single or Duplex PCR Single Reaction
Gel electrophoresis system MiniOne
Buffer TBE
DNA stain GelGreen
Gel images
Protocol notes

https://wolbachiaprojectdb.org/wp-content/uploads/2022/11/Arthropod-Identification-Lab-Protocol-and-Post-Lab.pdf

Used a DNA 10Kb ladder

Results
Wolbachia presence No
Confidence level High
Explanation of confidence level

Band present for Arth CO1, but not for Wolbachia. All controls worked as expected.

 

Not sent for sequencing
Wolbachia 16S sequence
Arthropod COI sequence
Summary The Stagmomantis was found to be negative for Wolbachia.
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