Sample information |
|
| Picture |
|
|---|---|
| Location | |
| Collection date | 09/15/2022 |
| Captive / Cultivated? | Wild-caught |
| Group | Edmund Burke School |
| Observations | Caught during summer, all other info n/a, as caught by different person. |
| Putative identification | Arthropoda Hexapoda Insecta Mantodea Mantidae Stagmomantis |
Methods |
|
| Extraction kit | DNeasy (Qiagen) |
| DNA extraction location | Gonads dissected |
| Single or Duplex PCR | Single Reaction |
| Gel electrophoresis system | MiniOne |
| Buffer | TBE |
| DNA stain | GelGreen |
| Gel images |
|
| Protocol notes | https://wolbachiaprojectdb.org/wp-content/uploads/2022/11/Arthropod-Identification-Lab-Protocol-and-Post-Lab.pdf Used a DNA 10Kb ladder |
Results |
|
| Wolbachia presence | No |
| Confidence level | High |
| Explanation of confidence level | Band present for Arth CO1, but not for Wolbachia. All controls worked as expected.
Not sent for sequencing |
| Wolbachia 16S sequence | |
| Arthropod COI sequence |
|
| Summary | The Stagmomantis was found to be negative for Wolbachia. |
Water Boatman