Sample information |
|
| Picture |
|
|---|---|
| Location | |
| Collection date | 10/05/2022 |
| Captive / Cultivated? | Wild-caught |
| Group | Edmund Burke School |
| Observations | |
| Putative identification | Arthropoda Crustacea Malacostraca Isopoda Armadillidae |
Methods |
|
| Extraction kit | DNeasy (Qiagen) |
| DNA extraction location | Abdomen |
| Single or Duplex PCR | Single Reaction |
| Gel electrophoresis system | MiniOne |
| Buffer | TBE |
| DNA stain | GelGreen |
| Gel images |
|
| Protocol notes | |
Results |
|
| Wolbachia presence | Unknown |
| Confidence level | Low |
| Explanation of confidence level | DNA sequencing results identified arthropod as mosquito, suspected DNA mix up during PCR. |
| Wolbachia 16S sequence | |
| Arthropod COI sequence |
|
| Summary | |
Extraction and Analysis of Wolbachia from Halyomorpha halys
Tick
Roly-Poly
Idaho Beetle
Grammonota