Drain Fly

November 22, 2022

Sample information
Picture
Photos by: Kayden V.
Location
Collection date 09/25/2022
Captive / Cultivated? Wild-caught
Group Edmund Burke School
Observations

Very small, black, 2 antennae, 2 wings, 6 small hairy legs.
Putative identification Arthropoda Hexapoda Insecta Diptera Psychodidae Clogmia Clogmia albipunctata

Methods
Extraction kit DNeasy (Qiagen)
DNA extraction location Whole arthropod
Single or Duplex PCR Single Reaction
Gel electrophoresis system MiniOne
Buffer TBE
DNA stain GelGreen
Gel images
Protocol notes

Results
Wolbachia presence No
Confidence level High
Explanation of confidence level

The first time we completed the gel electrophoresis lab, I accidentally didn’t add some of the primers, so nothing was showing up. However, we later redid it correctly, and our results displayed no Wolbachia, and the controls worked.
Wolbachia 16S sequence
Arthropod COI sequence Download FASTA    Download AB1
Summary The Clogmia albipunctata was found to be negative for Wolbachia.
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