Sample information |
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| Picture |
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|---|---|
| Location | |
| Collection date | 09/25/2022 |
| Captive / Cultivated? | Wild-caught |
| Group | Edmund Burke School |
| Observations | Very small, black, 2 antennae, 2 wings, 6 small hairy legs. |
| Putative identification | Arthropoda Insecta Diptera Psychodidae Clogmia Clogmia albipunctata |
Methods |
|
| Extraction kit | DNeasy (Qiagen) |
| DNA extraction location | Whole arthropod |
| Single or Duplex PCR | Single Reaction |
| Gel electrophoresis system | MiniOne |
| Buffer | TBE |
| DNA stain | GelGreen |
| Gel images |
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| Protocol notes |
|
Results |
|
| Wolbachia presence | No |
| Confidence level | High |
| Explanation of confidence level | The first time we completed the gel electrophoresis lab, I accidentally didn’t add some of the primers, so nothing was showing up. However, we later redid it correctly, and our results displayed no Wolbachia, and the controls worked. |
| Wolbachia 16S sequence | |
| Arthropod COI sequence | Download FASTA
Download AB1
|
| Summary | The Clogmia albipunctata was found to be negative for Wolbachia. |
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Wolbachia data
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