Sample information |
|
| Picture |
|
|---|---|
| Location | |
| Collection date | 09/14/2022 |
| Captive / Cultivated? | Wild-caught |
| Group | Woburn Memorial High School |
| Observations | -pea sized -hard shell |
| Putative identification | Arthropoda Crustacea Malacostraca Isopoda Armadillidae |
Methods |
|
| Extraction kit | Edwards Buffer |
| DNA extraction location | |
| Single or Duplex PCR | Duplex Reaction |
| Gel electrophoresis system | MiniOne |
| Buffer | TBE |
| DNA stain | GelGreen |
| Gel images |
|
| Protocol notes | 2% agarose gel. Well 1: Ladder (100bp) Well 2: Wolbachia (+) Well 3: Wolbachia (-) Well 4: Arthropod #1 Well 5: Arthropod #2 Well 6: Arthropod #3 Well 7: DNA control Well 8: dH2O Well 9: N/A |
Results |
|
| Wolbachia presence | Unknown |
| Confidence level | Medium |
| Explanation of confidence level | the positive arthropod DNA did not appear. |
| Wolbachia 16S sequence | |
| Arthropod COI sequence |
|
| Summary | |
Water Boatman