Wolbachia 2

March 30, 2023

Sample information
Picture
Photos by: Vanessa P.
Location
Collection date 09/14/2022
Captive / Cultivated? Wild-caught
Group Woburn Memorial High School
Observations

-size of a grain of rice.
Putative identification Arthropoda Insecta Hymenoptera Formicidae

Methods
Extraction kit Edwards Buffer
DNA extraction location
Single or Duplex PCR Duplex Reaction
Gel electrophoresis system MiniOne
Buffer TBE
DNA stain GelGreen
Gel images
Protocol notes

2% agarose gel.

Well 1: Ladder (100bp)

Well 2:  Wolbachia (+) 

Well 3:  Wolbachia (-)

Well 4:  Arthropod #1 (ant)

Well 5:  Arthropod #2 (pill bug)

Well 6: Arthropod #3 (pill bug)

Well 7: DNA control

Well 8: dH2O

Well 9: N/A

Results
Wolbachia presence Unknown
Confidence level Medium
Explanation of confidence level

the wolbachia positive DNA did not appear.
Wolbachia 16S sequence
Arthropod COI sequence
Summary
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