Sample information |
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| Picture |
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|---|---|
| Location | |
| Collection date | 09/14/2022 |
| Captive / Cultivated? | Wild-caught |
| Group | Woburn Memorial High School |
| Observations | Found it under a rock when the temperature of the local area was around 70-80 Fahrenheit. |
| Putative identification | Arthropoda Crustacea Malacostraca Isopoda Armadillidae |
Methods |
|
| Extraction kit | Edwards Buffer |
| DNA extraction location | Abdomen |
| Single or Duplex PCR | Single Reaction |
| Gel electrophoresis system | Edvotek Gel Electrophoresis |
| Buffer | TBE |
| DNA stain | GelGreen |
| Gel images |
|
| Protocol notes | Gel was moved a bit after having the DNA injected into the wells. |
Results |
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| Wolbachia presence | Yes |
| Confidence level | High |
| Explanation of confidence level | All of the controls, positive and negative, worked. If you look very closely there is a dim band closest to where a DNA band for Wolbachia would be expected |
| Wolbachia 16S sequence |
5'CAT ACC TAT TCG AAG GGA TAG 3' Forward, 5' AGC TTC GAG TGA AAC CAA TTC 3' Reverse
BLAST at The Wolbachia Project BLAST at NCBI
|
| Arthropod COI sequence |
5' GGT CAA CAA ATC ATA AAG ATA TTG G 3' Forward, 5' TAA ACT TCA GGG TGA CCA AAA AAT CA 3' Reverse
BLAST at The Wolbachia Project BLAST at NCBI
|
| Summary | The Armadillidae was found to be postive for Wolbachia. |
Water Boatman