|Captive / Cultivated?||Wild-caught|
|Group||Woburn Memorial High School|
Found it under a rock when the temperature of the local area was around 70-80 Fahrenheit.
|Putative identification||Crustacea Malacostraca Isopoda Armadillidae|
|Extraction kit||Edwards Buffer|
|DNA extraction location||Abdomen|
|Single or Duplex PCR||Single Reaction|
|Gel electrophoresis system||Edvotek Gel Electrophoresis|
Gel was moved a bit after having the DNA injected into the wells.
|Explanation of confidence level||
All of the controls, positive and negative, worked. If you look very closely there is a dim band closest to where a DNA band for Wolbachia would be expected
|Wolbachia 16S sequence||
5'CAT ACC TAT TCG AAG GGA TAG 3' Forward, 5' AGC TTC GAG TGA AAC CAA TTC 3' ReverseBLAST at The Wolbachia Project BLAST at NCBI
|Arthropod COI sequence||
5' GGT CAA CAA ATC ATA AAG ATA TTG G 3' Forward, 5' TAA ACT TCA GGG TGA CCA AAA AAT CA 3' ReverseBLAST at The Wolbachia Project BLAST at NCBI
|Summary||The Armadillidae was found to be postive for Wolbachia.|