DEMACASp23-16 Order:Hemiptera

September 7, 2023

Sample information
Picture
Photos by: Claudia Rosado
Location
Collection date 01/23/2023
Captive / Cultivated? Wild-caught
Group Georgia Southern University
Observations
  • Sweap net method
  • Georgia Southern University in the grass and bushes near the Student Success Center
  • Georgia Southern University in the grass across from the student parking lot by housing
Putative identification Arthropoda Hexapoda Insecta Hemiptera

Methods
Extraction kit
DNA extraction location Whole arthropod
Single or Duplex PCR Single Reaction
Gel electrophoresis system Standard electrophoresis system
Buffer TAE
DNA stain Other
Gel images
Protocol notes

DNA extraction kit of in-house reagents was used.

Results
Wolbachia presence No
Confidence level High
Explanation of confidence level

There was only one DNA band in our gel electrophoresis indicating positive insect DNA but no Wolbachia DNA.
Wolbachia 16S sequence
Arthropod COI sequence
Summary The Hemiptera was found to be negative for Wolbachia.
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