NESACBSp23-17-JA Hymenoptera

April 10, 2023

Sample information
Picture
Photos by: Justice A
Location No map location data available.
Collection date 11/15/2028
Captive / Cultivated? Wild-caught
Group Georgia Southern University
Observations
•Time of collection: Approximately 1:15 PM
•Environment of collection: Ant hill, Sunny, 57o F
•Method of collection: hand-in-jar
Putative identification Arthropoda Hexapoda Insecta Hymenoptera

Methods
Extraction kit
DNA extraction location Partial abdomen
Single or Duplex PCR Single Reaction
Gel electrophoresis system Standard electrophoresis system
Buffer TAE
DNA stain Other
Gel images
Protocol notes

DNA extraction kit of in-house reagents was used.

Results
Wolbachia presence No
Confidence level High
Explanation of confidence level

Local prevalence was approx. 9%. Controls worked in the experiment an can be verified with the gel image above
Wolbachia 16S sequence
Arthropod COI sequence
Summary The Hymenoptera was found to be negative for Wolbachia.
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