03A-MANOHEF22-01_CO1F

April 10, 2023

Sample information
Picture
Photos by: Joshua M
Location
Collection date 01/23/2023
Captive / Cultivated? Wild-caught
Group Georgia Southern University
Observations
Putative identification Arthropoda Hexapoda Insecta Coleoptera

Methods
Extraction kit
DNA extraction location Abdomen
Single or Duplex PCR Single Reaction
Gel electrophoresis system Standard electrophoresis system
Buffer TAE
DNA stain Other
Gel images
Protocol notes

DNA extraction kit of in-house reagents was used

Results
Wolbachia presence No
Confidence level High
Explanation of confidence level

Matched negative control.
Wolbachia 16S sequence
Arthropod COI sequence
CAGgaaTAGTAGGAACTTCATTAAGTATATTAATTCGAACTGAATtgcgt tcTCCTGGTGCATTGATTGGTAATGATCAAATTTATAATGTTATTGTTAC TGCTCATGCTTTCGTAATAATTTTTTTTATAGTTATACCTATTATAATTG GAGGATTTGGAAATTGATTAGTACCTTTAATATTAGGTGCTCCTGATATA GCCTTTCCTCGAATAAATAACATAAGTTTTTGATTACTTCCTCCTTCTTT AACTCTTCTCTTAATGAGAAGTATAGTGGAAAGAGGAGCTGGTACTGGAT GAACAGTTTACCCACCTTTATCATCTAGAATTGCTCATAGAGGAGCTTCA GTAGATTTAGCAATTTTTAGATTACATTTAGCTGGAATTTCATCAATTTT AGGTGCAGTAAACTTTATTACTACCATTATTAATATGCGATCAGTAGgAA TAAcatTTGATCGAATACCATTATTTGTATGAtCAgtAGGAATTACAGCA TTACTTTTACTTTTAtCATTACCAGtatTAGcaggaGCTATTActatatT ACTTACAGATcGAAATTTAAATACTT
BLAST at The Wolbachia Project   BLAST at NCBI
Summary The Coleoptera was found to be negative for Wolbachia.
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