Sample information |
|
| Picture |
|
|---|---|
| Location | |
| Collection date | 01/23/2023 |
| Captive / Cultivated? | Wild-caught |
| Group | Georgia Southern University |
| Observations | |
| Putative identification | Arthropoda Hexapoda Insecta Coleoptera |
Methods |
|
| Extraction kit | |
| DNA extraction location | Abdomen |
| Single or Duplex PCR | Single Reaction |
| Gel electrophoresis system | Standard electrophoresis system |
| Buffer | TAE |
| DNA stain | Other |
| Gel images |
|
| Protocol notes | DNA extraction kit of in-house reagents was used |
Results |
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| Wolbachia presence | No |
| Confidence level | High |
| Explanation of confidence level | Matched negative control. |
| Wolbachia 16S sequence | |
| Arthropod COI sequence |
CAGgaaTAGTAGGAACTTCATTAAGTATATTAATTCGAACTGAATtgcgt tcTCCTGGTGCATTGATTGGTAATGATCAAATTTATAATGTTATTGTTAC TGCTCATGCTTTCGTAATAATTTTTTTTATAGTTATACCTATTATAATTG GAGGATTTGGAAATTGATTAGTACCTTTAATATTAGGTGCTCCTGATATA GCCTTTCCTCGAATAAATAACATAAGTTTTTGATTACTTCCTCCTTCTTT AACTCTTCTCTTAATGAGAAGTATAGTGGAAAGAGGAGCTGGTACTGGAT GAACAGTTTACCCACCTTTATCATCTAGAATTGCTCATAGAGGAGCTTCA GTAGATTTAGCAATTTTTAGATTACATTTAGCTGGAATTTCATCAATTTT AGGTGCAGTAAACTTTATTACTACCATTATTAATATGCGATCAGTAGgAA TAAcatTTGATCGAATACCATTATTTGTATGAtCAgtAGGAATTACAGCA TTACTTTTACTTTTAtCATTACCAGtatTAGcaggaGCTATTActatatT ACTTACAGATcGAAATTTAAATACTT
BLAST at The Wolbachia Project BLAST at NCBI
|
| Summary | The Coleoptera was found to be negative for Wolbachia. |
Water Boatman