NESACBSp23-7 Diptera

Sample information

Picture
Photos by: M. Gonzalez
Location
Collection date
Captive / Cultivated? Wild-caught
Group Georgia Southern University
Observations
Putative identification Hexapoda Insecta Diptera

Methods

Extraction kit
DNA extraction location Whole arthropod
Single or Duplex PCR Single Reaction
Gel electrophoresis system Standard electrophoresis system
Buffer TAE
DNA stain Other
Gel images
Protocol notes

DNA extraction kit of in-house reagents was used.

 

Results

Wolbachia presence No
Confidence level Medium
Explanation of confidence level

I thought my insect was going to have Wolbachia¬†because I did all the steps correctly but the insect just didn’t have it.

Wolbachia 16S sequence
Arthropod COI sequence
Summary The Diptera was found to be negative for Wolbachia.
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