NESACBSp23-13-ZH Hymenoptera

April 10, 2023

Sample information
Picture
Photos by: Zachary H.
Location
Collection date 01/23/2023
Captive / Cultivated? Wild-caught
Group Georgia Southern University
Observations

Significantly larger than most other similar organisms, and had wings, most likely a mature adult.

Caught In between the Learning Commons and The International Gardens in front of a tree, within an anthill.

Weather was Sunny, 57 degrees Fahrenheit
Putative identification Arthropoda Hexapoda Insecta Hymenoptera

Methods
Extraction kit
DNA extraction location Abdomen
Single or Duplex PCR Single Reaction
Gel electrophoresis system Standard electrophoresis system
Buffer TAE
DNA stain Other
Gel images
Protocol notes

DNA extraction kit of in-house reagents was used.

Results
Wolbachia presence No
Confidence level High
Explanation of confidence level

Hymenoptera usually is not affected, additionally GEL image shows no sign of infection.
Wolbachia 16S sequence
Arthropod COI sequence
Summary The Hymenoptera was found to be negative for Wolbachia.
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