DEMACASP23-13JP Coleoptera

April 10, 2023

Sample information
Picture
Photos by: Janessa P.
Location No map location data available.
Collection date 01/25/2023
Captive / Cultivated? Wild-caught
Group Georgia Southern University
Observations

Caught in the field by the greenhouse.
Putative identification Arthropoda Hexapoda Insecta Coleoptera

Methods
Extraction kit
DNA extraction location Abdomen
Single or Duplex PCR Single Reaction
Gel electrophoresis system Standard electrophoresis system
Buffer TAE
DNA stain Other
Gel images
Protocol notes

A DNA extraction kit of in-house reagents was used.

Results
Wolbachia presence No
Confidence level Medium
Explanation of confidence level

I am confident in my results because I correctly followed the protocols and steps as well as my ability to identify whether or not the bands determined if my insect was infected or not, which it was not. I do have some doubts because the ladder did not transfer into the gel, which might have caused a disturbance.
Wolbachia 16S sequence
Arthropod COI sequence
Summary The Coleoptera was found to be negative for Wolbachia.
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