NESACKSp23-2-Coleoptera

April 13, 2023

Sample information
Picture
Photos by: Michaela P
Location
Collection date 01/26/2023
Captive / Cultivated? Wild-caught
Group Georgia Southern University
Observations
•Time: 10:50 am
•Location : the lower east side of the pond on the Armstrong Campus
•Environment :  A tree
•Collection Method : Canvas and Beating Stick
Putative identification Arthropoda Hexapoda Insecta Coleoptera

Methods
Extraction kit
DNA extraction location Whole arthropod
Single or Duplex PCR Single Reaction
Gel electrophoresis system Standard electrophoresis system
Buffer TAE
DNA stain Other
Gel images
Protocol notes

DNA extraction kit of in-house reagents was used.

Results
Wolbachia presence No
Confidence level High
Explanation of confidence level

The controls indicated that my insect does not contain the Wolbachia 16SrRNA gene.
Wolbachia 16S sequence
Arthropod COI sequence
Summary The Coleoptera was found to be negative for Wolbachia.
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