NESACKSp23-4-BP Hymenoptera

April 13, 2023

Sample information
Picture
Photos by: Briana Phillips
Location
Collection date 01/26/2023
Captive / Cultivated? Wild-caught
Group Georgia Southern University
Observations

Collection was taken on the east side of science center. The collection method was with tweezers and by hand. We found specimen under logs. The weather was overcast, windy and cold.
Putative identification Arthropoda Hexapoda Insecta Hymenoptera

Methods
Extraction kit
DNA extraction location Whole arthropod
Single or Duplex PCR Single Reaction
Gel electrophoresis system Standard electrophoresis system
Buffer TAE
DNA stain Other
Gel images
Protocol notes

DNA extraction kit of in-house reagents was used.

Results
Wolbachia presence No
Confidence level Medium
Explanation of confidence level

I completed all labs to the best of my ability. I feel completing them appropriately and effectively allows me to have confidence in the results found based on local prevalence data.
Wolbachia 16S sequence
Arthropod COI sequence
Summary The Hymenoptera was found to be negative for Wolbachia.
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