Sample information |
|
| Picture |
|
|---|---|
| Location | |
| Collection date | 01/26/2023 |
| Captive / Cultivated? | Wild-caught |
| Group | Georgia Southern University |
| Observations | Collection was taken on the east side of science center. The collection method was with tweezers and by hand. We found specimen under logs. The weather was overcast, windy and cold. |
| Putative identification | Arthropoda Hexapoda Insecta Hymenoptera |
Methods |
|
| Extraction kit | |
| DNA extraction location | Whole arthropod |
| Single or Duplex PCR | Single Reaction |
| Gel electrophoresis system | Standard electrophoresis system |
| Buffer | TAE |
| DNA stain | Other |
| Gel images |
|
| Protocol notes | DNA extraction kit of in-house reagents was used. |
Results |
|
| Wolbachia presence | No |
| Confidence level | Medium |
| Explanation of confidence level | I completed all labs to the best of my ability. I feel completing them appropriately and effectively allows me to have confidence in the results found based on local prevalence data. |
| Wolbachia 16S sequence | |
| Arthropod COI sequence |
|
| Summary | The Hymenoptera was found to be negative for Wolbachia. |
Water Boatman