Hymenoptera

April 14, 2023

Sample information
Picture
Photos by: Annie N
Location
Collection date 01/25/2023
Captive / Cultivated? Wild-caught
Group Georgia Southern University
Observations

Location: The Pond (North)

Date: 1/25/2023

Time: Afternoon 1:10 pm

Weather: Warm/Cloudy/50 degree

Habitat: Sand Area Near The Pond
Putative identification Arthropoda Hexapoda Insecta Hymenoptera

Methods
Extraction kit
DNA extraction location Whole arthropod
Single or Duplex PCR Single Reaction
Gel electrophoresis system Standard electrophoresis system
Buffer TAE
DNA stain Other
Gel images
Protocol notes

DNA extraction kit of in-house reagents was used

Results
Wolbachia presence No
Confidence level High
Explanation of confidence level

I’m pretty confident that my insect results coming back negative are correct due to the followed direction of my partner and I during the extraction process.
Wolbachia 16S sequence
GaccCGAGAACGTATTCACCGTGGCATGCTGATCCACGATTACTAGCGAT TcccactTCATGCACTCGAGTTGCAGAGTACAATCCGAACTGAGATGTCT TTTAGGGATTAGCTTAGGCTTGCGCACCTTGCAGCCCATTGTAGACACCA TTGTAGCACGTGTGTAGCCCACTTCATAAAGGCCATGATGACTTGACATC ATCCCCATCTTCCTCCAGCTTATCACTGGCAGTTTCCTTAAAGTGCTCAG CATTACCTGATGGCAACTAAGGATGAGGGTTGCGCTCGTTGCGGGACTTA ACCCAACATCTCACGACACGAGCTGACGACAGCCATGCAACACCTGTGTA AAATCCGGCCGAACCGACCCTATCCCTTCGAATAGGTAT
BLAST at The Wolbachia Project   BLAST at NCBI
Arthropod COI sequence
CGTGCTGATCCACGATTACTAGCGATTcctatttcATGCACTCGAGTTGC AGAGTACAATCCGAACTGAGATGGCTTTTAAGGGATTAGCTTAGCCTCGC GACTTTGCAGCCCATTGTAGCCACCATTGTAGCACGTGTGTAGCCCACTC CATAAGGGCCATGATGACTTGACATCATCCCCACCTTCCTCCAgttTATC ACTGGCAGTTTCCTTAAAGTCCCCAGCATTACCTGATGGTAACTAAGGAT GAGGGTTGCGCTCGTTGCGGGACTTAACCCAACATCTCACGACACGAGCT GACGACAGCCATGCAACACCTGTGTGAAATCCGGCCGAACCGACCCTATC CCTT
BLAST at The Wolbachia Project   BLAST at NCBI
Summary The Hymenoptera was found to be negative for Wolbachia.
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