ZETACESp23-9 Odonata

April 15, 2023

Sample information
Picture
Photos by: Gabrielle S
Location No map location data available.
Collection date 01/24/2023
Captive / Cultivated? Wild-caught
Group Georgia Southern University
Observations

Savannah, Ga, ponds at GSU Armstrong Campus. Used a D-net, scooping method. Sunny, dry, and cold. Took many tries to find anything.
Putative identification Arthropoda Hexapoda Insecta Odonata

Methods
Extraction kit
DNA extraction location Abdomen
Single or Duplex PCR Single Reaction
Gel electrophoresis system Standard electrophoresis system
Buffer TAE
DNA stain Other
Gel images
Protocol notes

DNA extraction kit of in-house reagents was used.

Results
Wolbachia presence Yes
Confidence level High
Explanation of confidence level

I am positive my insect was positive because after gel electrophoresis, my insect had the same number of bands as my positive control.
Wolbachia 16S sequence
Arthropod COI sequence
Summary The Odonata was found to be postive for Wolbachia.
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