Sample information |
|
| Picture |
|
|---|---|
| Location | No map location data available. |
| Collection date | 01/24/2023 |
| Captive / Cultivated? | Wild-caught |
| Group | Georgia Southern University |
| Observations | Savannah, Ga, ponds at GSU Armstrong Campus. Used a D-net, scooping method. Sunny, dry, and cold. Took many tries to find anything. |
| Putative identification | Arthropoda Insecta Odonata |
Methods |
|
| Extraction kit | |
| DNA extraction location | Abdomen |
| Single or Duplex PCR | Single Reaction |
| Gel electrophoresis system | Standard electrophoresis system |
| Buffer | TAE |
| DNA stain | Other |
| Gel images |
|
| Protocol notes | DNA extraction kit of in-house reagents was used. |
Results |
|
| Wolbachia presence | Yes |
| Confidence level | High |
| Explanation of confidence level | I am positive my insect was positive because after gel electrophoresis, my insect had the same number of bands as my positive control. |
| Wolbachia 16S sequence | |
| Arthropod COI sequence |
|
| Summary | The Odonata was found to be postive for Wolbachia. |
Common Eastern Bumble Bee (Bombus impatiens)
American Bird
Spotted crane fly
Wolbachia data
Meadow Katydid