Sample information |
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Picture |
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Location | No map location data available. |
Collection date | |
Captive / Cultivated? | Wild-caught |
Group | Bordenstein Lab |
Observations | Sample 85 |
Putative identification | Arthropoda Hexapoda Insecta Diptera Muscidae Phaonia Phaonia angelicae |
Methods |
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Extraction kit | |
DNA extraction location | |
Single or Duplex PCR | |
Gel electrophoresis system | |
Buffer | |
DNA stain | |
Gel images |
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Protocol notes | |
Results |
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Wolbachia presence | Yes |
Confidence level | High |
Explanation of confidence level | Possible Wolbachia co-infection |
Wolbachia 16S sequence | Download FASTA
Download AB1
GTGTTGCATGGCTGTCGTCAGCTCGTGTCGTGAGATGTTGGGTTAAGTCCCGCAACGAGCGCAACCCTCATCCTTAGTTACCATCAGGTAATGCTGGGGACTTTAAGGAAACTGCCAGTGATAAACTGGAGGAAGGTGGGGATGATGTCAAGTCATCATGGCCCTTATGGAGTGGGCTACACACGTGCTACAATGGTGGCTACAATGGGCTGCAAAGTGCCCAGGCTAAACCAATCCCTTAAAAGACATCTCAGTTCGGATTGTACTCTGCAACTCGAGTGCATGAAGTTGGAATCGCTAGTAATCGTGGATCAGCATGCCACGGTGAATACGTTCTCGGGTCTTGTACACACTGCCCGTCACGCCATGGGAATTGGT
BLAST at The Wolbachia Project BLAST at NCBI
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Arthropod COI sequence | Download FASTA
Download AB1
TTTTTGGTGCTTGATCAGGAATAGTAGGAACTTCACTAAGTATTCTAATTCGAGCCGAATTAGGACACCCTGGAGCTTTAATTGGAGATGATCAAATTTATAATGTAATCGTAACAGCTCATGCTTTTGTTATAATTTTTTTTATAGTTATACCTATTATAATTGGAGGATTTGGAAATTGACTAGTGCCCCTAATATTAGGAGCCCCAGACATAGCCTTTCCTCGAATAAATAATATGAGATTTTGACTTTTACCTCCTGCTTTAACATTGCTGCTAACTAGCAGCATAGTAGAAACTGGAGCTGGAACAGGATGAACTGTTTACCCCCCACTATCATCTAATATTGCTCATGGAGGAGCCTCTGTTGATTTAGCTATTTTTTCCCTTCATTTAGCAGGAATCTCCTCTATTCTAGGAGCCGTAAATTTTATTACAACTGTAATTAATATACGATCAATAGGAATTACATTCGATCGCATACCTTTATTTGTATGATCAGTAGTAATTACAGCTTTATTATTACTTTTATCATTACCTGTTTTAGCAGGAGCTATTACAATATTATTGACAGATCGAAATTTAAATACATCTTTTTTTGA
BLAST at The Wolbachia Project BLAST at NCBI
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Summary | The Phaonia angelicae was found to be postive for Wolbachia. |