Sample information |
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| Picture |
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|---|---|
| Location | |
| Collection date | 01/25/2023 |
| Captive / Cultivated? | Wild-caught |
| Group | Georgia Southern University |
| Observations | Date of collection3: 1/25/2023 Time of collection3: 1:12 PM Weather conditions3: Cool, gloomy day- right before rain
Location of collection3: Outside of Science Center and Greenhouse on Georgia Southern Armstrong Campus Environment of collection (habitats)5: Bushes, Trees, dirt, under tree branches on the ground, open area of grass Method of collection5: 3 methods of collection
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| Putative identification | Arthropoda Hexapoda Insecta Hemiptera |
Methods |
|
| Extraction kit | |
| DNA extraction location | Whole arthropod |
| Single or Duplex PCR | Single Reaction |
| Gel electrophoresis system | Standard electrophoresis system |
| Buffer | TAE |
| DNA stain | Other |
| Gel images |
|
| Protocol notes | DNA extraction kit of in-house reagents was used. |
Results |
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| Wolbachia presence | No |
| Confidence level | Medium |
| Explanation of confidence level | When loading my wells, the samples disappeared while waiting. They did not show up during the process either, even though i loaded to wells correctly. |
| Wolbachia 16S sequence | |
| Arthropod COI sequence |
|
| Summary | The Hemiptera was found to be negative for Wolbachia. |
Millipede
Cylindroiulus caeruleocinctus
Biting Midge
Eastern Red Centipede
Specimen_3