Sample information |
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Picture |
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Location | |
Collection date | 01/25/2023 |
Captive / Cultivated? | Wild-caught |
Group | Georgia Southern University |
Observations | Date of collection3: 1/25/2023 Time of collection3: 1:12 PM Weather conditions3: Cool, gloomy day- right before rain
Location of collection3: Outside of Science Center and Greenhouse on Georgia Southern Armstrong Campus Environment of collection (habitats)5: Bushes, Trees, dirt, under tree branches on the ground, open area of grass Method of collection5: 3 methods of collection
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Putative identification | Arthropoda Hexapoda Insecta Hemiptera |
Methods |
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Extraction kit | |
DNA extraction location | Whole arthropod |
Single or Duplex PCR | Single Reaction |
Gel electrophoresis system | Standard electrophoresis system |
Buffer | TAE |
DNA stain | Other |
Gel images |
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Protocol notes | DNA extraction kit of in-house reagents was used. |
Results |
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Wolbachia presence | No |
Confidence level | Medium |
Explanation of confidence level | When loading my wells, the samples disappeared while waiting. They did not show up during the process either, even though i loaded to wells correctly. |
Wolbachia 16S sequence | |
Arthropod COI sequence |
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Summary | The Hemiptera was found to be negative for Wolbachia. |