MLSZ123456

May 12, 2023

Sample information
Picture
Photos by: Michelle L & Shuai Z
Location
Collection date 04/06/2023
Captive / Cultivated? Wild-caught
Group Arroyo High School
Observations

The spider is hairy and had a big stomach. We found the spider dead in the bricks. It also had dirt all over the legs.
Putative identification Arthropoda Chelicerata Arachnida Araneae

Methods
Extraction kit DNeasy (Qiagen)
DNA extraction location Abdomen
Single or Duplex PCR Single Reaction
Gel electrophoresis system Standard electrophoresis system
Buffer TAE
DNA stain SYBR Safe
Gel images
Protocol notes

Results
Wolbachia presence Unknown
Confidence level Low
Explanation of confidence level

We aren’t confident in our spider being Wolbachia positive since no bands showed up for both Wolbachia gels we did.
Wolbachia 16S sequence
Arthropod COI sequence
Summary
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