Sample information |
|
| Picture |
|
|---|---|
| Location | |
| Collection date | 04/06/2023 |
| Captive / Cultivated? | Wild-caught |
| Group | Arroyo High School |
| Observations | The spider is hairy and had a big stomach. We found the spider dead in the bricks. It also had dirt all over the legs. |
| Putative identification | Arthropoda Chelicerata Arachnida Araneae |
Methods |
|
| Extraction kit | DNeasy (Qiagen) |
| DNA extraction location | Abdomen |
| Single or Duplex PCR | Single Reaction |
| Gel electrophoresis system | Standard electrophoresis system |
| Buffer | TAE |
| DNA stain | SYBR Safe |
| Gel images |
|
| Protocol notes | |
Results |
|
| Wolbachia presence | Unknown |
| Confidence level | Low |
| Explanation of confidence level | We aren’t confident in our spider being Wolbachia positive since no bands showed up for both Wolbachia gels we did. |
| Wolbachia 16S sequence | |
| Arthropod COI sequence |
|
| Summary | |
Water Boatman