SDRV7 Ladybug Wolbachia

May 15, 2023

Sample information
Picture
Photos by: Rafael V.
Location
Collection date 04/06/2023
Captive / Cultivated? Wild-caught
Group Arroyo High School
Observations
Putative identification Arthropoda Hexapoda Insecta Coleoptera Coccinellidae

Methods
Extraction kit DNeasy (Qiagen)
DNA extraction location body without wings
Single or Duplex PCR Single Reaction
Gel electrophoresis system Standard electrophoresis system
Buffer TAE
DNA stain SYBR Safe
Gel images
Protocol notes

Results
Wolbachia presence No
Confidence level Medium
Explanation of confidence level

We only had one ladybug to work with so I believe that was insufficient enough to not produce any results. If they did have Wolbachia it would have shown since from the gel we can see that the DNA ladder did in fact work as intended.
Wolbachia 16S sequence
Arthropod COI sequence
Summary The Coccinellidae was found to be negative for Wolbachia.
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